Composite

Part:BBa_K2142005:Design

Designed by: Lukas Rieder   Group: iGEM16_NAWI-Graz   (2016-10-07)


Composite part containing inducible mazF toxin and dam metylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part had to be inducible. Later we figured out that it was not that smart to use the same RBS because E.coli did recombination.


Source

We let it synthesize

References